Activity of Desulfovibrio sp and Thiobacillus thioparus sp On Extra Polymeric Sub- stance Produced by the Combination of Pseudomonas sp With E-coli

The main intention of this study to understand the microbial consortium of Sulphate Reducing Bacteria(SRB) and Sulphure oxidizing bacteria (SOB) on Extra polymeric substance produced by Pseudomonas sp., with combination of E-coli. In stressing the importance of anaerobic corrosion by SRB, corrosive effects of acids produced by microorganisms may be overlooked Sulphur oxidizers chiefly of the genus Thiobacillus sp., which is a strongly corrosive agent to be studied to understand their activity of SOB and SRB in Microbial Influenced Corrosion. The activity of Sulphate reducing bacteria and Sulphate oxidizing bacteria on biofilm produced by two different combinations of extra polymeric substance (EPS) excreting organisms are discussed in this paper. Microbial Conditioning of Galvanised iron pipes and Remnants Samples (1.5cm x 1.5cm) prepared and introduced in to different sets of aquatic environment. EPS were estimated quantitatively by Micheal Duboisis and Lowery modified method. The activities of SRB and SOB measured by estimating the EPS, before inoculation and after inoculation of SRB and SOB.The result of EPS estimation exposed that the Desulfovibrio sp.,(SRB) enhance the efficiency of Pseudomonas aeruginosa and E-Coli for EPS secretion. After the inoculation of Thiobacillus thioparus(SRB) the slope of EPS step downed and this organisms populated in every in a week of experimental course. The sulphur reducing organism leachate the EPS, or may be consumed for further colonization. The comparative study of Pseudomonas fluorescence and Pseudomonas aeruginosa SEM analysis revealed that Pseudomonas fluorescence secrets the more EPS than Pseudomonas aeruginosa along with combination of E-Coli. The SRB’s are enhances the excretion of polymers (Glycogalyx) along with Biofilm forming bacteria by inducing symbiotic relations. The significant of this study is revealed the role of SRB and SOB with association with biofilm forming bacteria in microbial induced corrosion. SRB ‘s enhances the EPS secretion in massive level and SOB’s are consuming the EPS as a energy source for the production acid base products lead to metal corrosion in aquatic environment. .